Search results for "Ige reactivity"

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Comparison of ammoniated and nonammoniated extracts in children with latex allergy

2003

Background:  The use of ammoniated or nonammoniated latex extracts for the diagnosis of latex allergy is still a matter of debate. The aim of our study was to compare the characteristics of the two types of extracts by immunoblotting and RAST techniques in children with ascertained latex allergy. Methods:  Ammoniated (AL) and nonammoniated latex (NAL) extracts were prepared and blotted on SDS-PAGE to resolve their components. Also a solid phase for RAST assays was prepared with the two extracts. The sera from 18 children (mean age 11.4 years, range 6–15 years), with ascertained latex allergy (clinical history, skin test, CAP-RAST and provocation) were used for the experiments. Results:  The…

MaleAllergyLatex HypersensitivityAdolescentLatexImmunologyProvocation testImmunoblottingmedicine.disease_causeImmunoglobulin ESensitivity and SpecificityIge reactivityAllergenRadioallergosorbent TestAmmoniaLatex HypersensitivitymedicineImmunology and AllergyHumansChildSensitivity and Specificity; Immunoblotting; Electrophoresis Polyacrylamide Gel; Latex Hypersensitivity; Skin Tests; Ammonia; Humans; Child; Latex; Radioallergosorbent Test; Allergens; Immunoglobulin E; Adolescent; Female; Maleammoniated extract; diagnosis; immunoblotting; latex allergy; nonammoniated extract; RASTSkin Testsbiologymedicine.diagnostic_testSkin TestChemistryRadioallergosorbent testAllergenAllergensImmunoglobulin Emedicine.diseaseLatex allergyImmunologybiology.proteinElectrophoresis Polyacrylamide GelFemaleHuman
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